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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Clin Exp Allergy. 2019 Nov 1;50(1):121–124. doi: 10.1111/cea.13512

Figure 1: Delayed TSLP induction by DEP stimulated bronchial epithelial cells.

Figure 1:

HBECs were grown in 6 well plates to 90% confluence and starved over-night before stimulation with 0.2, 1 or 5μg/cm2 of DEP (A). Media was removed and replaced with TRIZOL 3h30 and 24h later. (A) CYP1A1 and HMOX-1 mRNA levels; (B) IL-1β and IL-6 mRNA levels and (C) TSLP and IL-8 mRNA levels were determined by real time quantitative PCR and normalized to GAPDH. (D) IL-1β, IL-8 and TSLP mRNA levels following exposure to 5μg/cm2 of DEP, 25μg/ml of HDM or both (data compiled from two separate experiments).