Figure 5.

Efficient Transduction of Healthy Human T Cells by SJ293TS-Derived Lentiviral Vector

CD4⁺ and CD8⁺ human T cells from a single healthy donor were transduced with four separate lentiviral vector preparations derived from SJ293TS cells consisting of two different anti-CD123-CAR lentiviral vectors at a MOI of 25. (A–C) Seven days post transduction, the CD123 CAR expression was determined by flow cytometry analysis (A); the total number of cells, viability (B), and vector copy numbers (C) were also measured.