Table 2.

Linearity, LOD and LOQ data of the 25 analytes by UPLC–MS/MS.

Analytesa	Calibration curves	R2	Range (ng/mL)	LOD (ng/mL)	LOQ (ng/mL)
1	y = 4.694 × 107 x – 2.844 × 104	0.9960	14.77–3781	1.18	3.58
2	y = 2.207 × 109 x + 3.386 × 105	0.9978	21.97–5625	0.25	0.76
3	y = 1.409 × 108 x – 1.767 × 104	0.9975	13.42–3437	1.43	4.33
4	y = 5.046 × 108 x – 1.526 × 105	0.9997	12.93–3312	0.34	1.03
5	y = 9.617 × 109 x – 3.25 × 106	0.9933	12.57–3218	0.06	0.18
6	y = 4.699 × 108 x – 9.081 × 105	0.9953	14.52–3718	0.70	2.12
7	y = 9.332 × 109 x – 1.006 × 105	0.9961	9.03–2312	0.11	0.33
8	y = 1.022 × 109 x – 2.396 × 104	0.9995	15.50–3968	0.28	0.85
9	y = 5.724 × 109 x + 5.254 × 104	0.9999	11.84–3031	0.13	0.39
10	y = 1.155 × 109 x + 7.420 × 104	0.9684	13.79–3531	0.09	0.27
11	y = 2.424 × 108 x – 2.028 × 105	0.9984	11.96–3062	0.05	0.15
12	y = 2.565 × 109 x – 1.232 × 105	0.9985	33.81–8656	0.21	0.64
13	y = 4.571 × 108 x – 1.569 × 105	0.9930	13.54–3468	0.91	2.76
14	y = 2.565 × 109 x – 1.232 × 105	0.9956	9.89–2531	0.17	0.52
15	y = 6.865 × 108 x – 2.800 × 105	0.9992	15.01–3843	0.33	1.00
16	y = 7.750 × 105 x + 7.177 × 102	0.9944	11.59–2968	1.68	5.09
17	y = 1.221 × 109 x – 7.151 × 105	0.9982	16.11–4125	0.43	1.30
18	y = 9.945 × 108 x – 5.402 × 105	0.9995	12.08–3093	0.50	1.52
19	y = 9.139 × 106 x + 3.203 × 104	0.9985	15.38–3937	1.31	3.97
20	y = 4.601 × 108 x + 1.885 × 104	0.9968	15.01–3843	0.12	0.36
21	y = 2.174 × 108 x + 2.735 × 104	0.9924	14.03–3593	0.88	2.67
22	y = 8.741 × 107 x + 2.657 × 104	0.9973	13.54–3468	0.05	0.15
23	y = 1.051 × 106 x – 8.457 × 102	0.9990	15.99–4093	1.94	5.88
24	y = 2.529 × 108 x – 1.173 × 105	0.9977	15.38–3937	0.17	0.52
25	y = 2.539 × 109 x – 1.450 × 106	0.9957	11.35–2906	0.36	1.09

^aThe No. of analytes is the same as that in Table 1.