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. 2019 Oct 29;68(1):59–72. doi: 10.1369/0022155419886263

Figure 5.

Figure 5.

Effects of knockdown of LSR, TRIC, ASPP2, PAR3, and YAP in Sawano cells. (A) Immunocytochemical staining for LSR (green), TRIC (green), ASPP2 (green), PAR3 (green), and pYAP (green) with acetylated tubulin (red) in Sawano cells treated with 100 ng/ml Taxol after transfection with siRNAs of LSR, TRIC, ASPP2, PAR3, and pYAP. Bars: 5 μm. (B) Immunocytochemical staining for LSR, TRIC, ASPP2, PAR3, and pYAP with γ-tubulin in Sawano cells treated with 100 ng/ml Taxol after transfection with siRNAs of LSR, TRIC, ASPP2, PAR3, and pYAP. Bars: 5 μm. (C) Bar graph of cell cycle assay in Sawano cells transfected with siRNA of LSR. (D) Bar graph of cell cycle assay in Sawano cells transfected with siRNA of ASPP2. Abbreviations: LSR, lipolysis-stimulated lipoprotein receptor; TRIC, tricellulin; ASPP2, apoptosis-stimulating of p53 protein 2; pYAP, phosphorylated yes-associated protein; OCLN, occludin; CLDN-7, claudin-7; ZO-1, zonula occludens-1; siRNA, small interfering RNA.