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. 2019 Oct 29;68(1):59–72. doi: 10.1369/0022155419886263

Figure 6.

Figure 6.

Tight junction molecules ASPP2 and pYAP are colocalized with Hook2 in centrosomes during cytokinesis in Sawano cells. (A) Immunocytochemical staining for acetylated tubulin, γ-tubulin, LSR, ASPP2, and OCLN with Hook2 in Sawano cells treated with 100 ng/ml Taxol for 24 hr. Bars: 5 μm. (B) Western blotting for pYAP, TRIC, LSR, PAR3, and ASPP2 in immunoprecipitates with anti-Hook2 antibody in Sawano cells treated with 100 ng/ml Taxol for 24 hr. Abbreviations: ASPP2, apoptosis-stimulating of p53 protein 2; pYAP, phosphorylated yes-associated protein; LSR, lipolysis-stimulated lipoprotein receptor; OCLN, occludin; TRIC, tricellulin.