Skip to main content
. 2019 Oct 29;68(1):59–72. doi: 10.1369/0022155419886263

Figure 7.

Figure 7.

Effects of knockdown of Hook2 in centrosomes during cytokinesis induced by Taxol in Sawano cells. (A) Immunocytochemical staining for Hook2, LSR, TRIC, OCLN, CLDN-7, ZO-1, cingulin, and pYAP with γ-tubulin in Sawano cells treated with 100 ng/ml Taxol after transfection with siRNA of Hook2. Bars: 5 μm. (B) Western blot analysis of Hook2, LSR, TRIC, CLDN-7, cingulin, pYAP, and γ-tubulin in Sawano cells treated with or without 100 ng/ml Taxol after transfection with siRNA of Hook2. (C) Graph of TEER values representing barrier function of Sawano cells transfected with siRNA of Hook2. (D) Graph of TEER values representing barrier function of Sawano cells treated with 100 ng/ml Taxol after transfection with siRNA of Hook2. Abbreviations: LSR, lipolysis-stimulated lipoprotein receptor; TRIC, tricellulin; OCLN, occludin; CLDN-7, claudin-7; ZO-1, zonula occludens-1; pYAP, phosphorylated yes-associated protein; TEER, transepithelial electrical resistance; siRNA, small interfering RNA.