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. 2019 Dec 23;13(2):287–294. doi: 10.1016/j.tranon.2019.09.011

Figure 2.

Figure 2

PBK promoted GBM tumorigenesis both in vitro and in vivo. (A) The mRNA expression level of PBK in GBM cells after transfection with shRNA against PBK (shPBK) or nontargeting control (shNT). (B) The protein expression level of PBK in GBM cells after transfection with shPBK or shNT. β-Actin served as the control. (C) Representative phase-contrast and fluorescence images of cell cloning assay in GBM cells transfected with shPBK and shNT. (D) Cell cloning assay showed reduced cell cloning numbers after knock-down of PBK in GBM cells. (E) The cell proliferation rate was remarkably inhibited by knockdown of PBK expression in GBM cells. (F) Representative H&E staining of brain sections of intracranial xenograft mice. (G) Kaplan–Meier analysis showed longer survival of xenograft mice implanted with shPBK GBM cells compared with control. **P < 0.01.