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. 2019 Dec 21;13(2):221–232. doi: 10.1016/j.tranon.2019.09.013

Figure 2.

Figure 2

Simultaneous inhibition of BRD4 and PLK1 synergistically represses MYC/N and results in increased cell death. A Fluorescence-activated cell sorting (FACS)-based measurement of IMR5 cell cycle distribution after treatment with DMSO control, MK-8628 (150 nM), Volasertib (5 nM), or combination of both agents (150 nM MK-8628 + 5 nM Volasertib) for 72 h. B Fraction of cells in S-phase as measured in (A). C Fraction of cells in Sub G1 phase as measured in A (A). D Western immunoblotting of MYCN and cleaved caspase 3 after treatment of cells with DMSO control, MK-8628 (500 nM), Volasertib (10 nM), or combination of both (500 nM MK-8628 + 10 nM Volasertib) (Student's t-test: * = P < 0.05, ** = P < 0.01, *** = P < 0.001, **** = P < 0.0001).