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. 2019 Dec 21;13(2):221–232. doi: 10.1016/j.tranon.2019.09.013

Figure 4.

Figure 4

Treatment with dual BRD4/PLK1 inhibitors UMB103 and UMB160 leads to cell death and cell cycle arrest in pediatric tumor cell lines. A FACS-based measurement of the cell cycle distribution of IMR5 cells after treatment with DMSO, UMB103 (10 nM) or UMB160 (10 nM) for 72 hours. B Fraction of cells in S-phase as measured in (A). C Fraction of cells in Sub G1 phase as measured in (A). D Western immunoblotting of cleaved caspase 3 after incubation of cells with DMSO compared with UMB103 and UMB160 for 24 hours at 20 nM each (Student's t-test: * = ns = not significant, P < 0.05, ** = P < 0.01, *** = P < 0.001, **** = P < 0.0001).