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. 2019 Dec 19;13(2):177–185. doi: 10.1016/j.tranon.2019.10.015

Figure 2.

Figure 2

Analysis of dietary lipid uptake in kidney cortices of transgenic TRACK mice compared with wild-type mice. Cortices from six TRACK and five WT mouse kidneys were compared for RNA expression and small molecule intermediates and products of metabolism (metabolomics). Statistical significance was assessed using a two-sided Student's t-test. A illustrates the specific lipid biosynthesis (left panel), lipid degradation (center panel), and lipid uptake (right panel) products analyzed. B illustrates quantification of mRNA (Fragments Per Kilobase per Million mapped reads [FPKM]) comparing WT and TRACK kidney cortices. Transcripts related to lipid biosynthesis, uptake, and storage are increased, while the mRNA levels of lipid degradation genes are reduced in TRACK compared with WT. C illustrates the relative ion abundance of metabolites. Note a significant (p < 0.05) increase in the levels of (essential) fatty acids (palmitoleic acid, oleic acid, linoleic acid, alpha linolenic acid), as well as a large (p < 0.05) decrease in fatty acid carnitine products (oleoylcarnitine) in TRACK as compared with WT kidneys, showing that HIF1α promotes the uptake of dietary lipids (such as the essential fatty acids linoleic and alpha-linolenic acid) presumably through increased expression of lipid receptors in the kidneys of transgenic mice.