Figure 3.

Impact of a high fat-diet on lipid accumulation in the kidneys of TRACK transgenic mice compared with wild-type mice. Mice (43 total) were fed a high-fat diet (HFD) (TRACK n = 23, wild-type n = 20), and 24 mice were fed with regular chow diet (TRACK n = 16, wild-type n = 8). A illustrates the total body weight of individual mice at baseline, 6 and 12 weeks on the respective diets. Both TRACK and WT mice fed a HFD showed increased body weights compared with chow-fed mice (p < 0.05). B shows representative H&E stained sections of kidney cortex of WT and TRACK mice fed a regular chow (left panels) or HFD (right panels). The scale bar indicates 100 μm. Note the increase in clear cell lipid containing vacuoles in TRACK mice fed a HFD, confirmed by staining mature lipids with Oil Red O (ORO) (C).