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. 2019 Dec 26;15:157. doi: 10.1186/s13007-019-0542-5

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© The Author(s) 2019

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Workflow of a VIGS experiment a after identification of gene sequences and validation by sequencing, suitable siRNAs and off-targets are predicted. Based on these predictions silencing gene fragments were defined. b For in vivo validation silencing fragments are cloned in the VIGS-vectors and transformed in Agrobacteria for infiltration experiments