Fig. 3.

Silencing of PDS induced with the Cotton leaf crumple virus a Plants were infiltrated with the A. tumefaciens strain AGL1 carrying pCotton-PDS or an empty-vector control. Phenotypes could be identified approximately four weeks after infiltration with pCotton-PDS (right), whereas control plants showed no phenotype (left). b Overview on the efficiency of VIGS with different A. tumefaciens strains and number of infiltrated plants. c Quantification of the expression level of PDS in plants showing a phenotype and empty- vector control plants were analyzed by qPCR and normalized relative to the expression levels of eIFa and UBQ5. Error bars indicate standard error of five biological replicates. d Quantification of pigment content (chlorophyll a, chlorophyll b and carotenoids) of leaves from empty-vector control plants and PDS-silenced plants. Error bars indicate standard error of 3 representative replicates