FIG 1.
Behavior of single motors of E. coli strains. Representative motors from E. coli HCB5 (AW405 fliCsticky) and isogenic ycgR, yhjH, and ycgR yhjH mutants were monitored by recording the motion of polystyrene beads attached to filament stubs (see Materials and Methods). Counterclockwise (CCW) and clockwise (CW) speeds are shown. Average CCW speeds (expressed in Hz, unit of frequency that refers to the number of rotations per second) are 76 ± 14, 78.7 ± 14, 56 ± 8, and 83.6 ± 15, and switching frequencies (reversals in motor direction per minute, i.e., when the trace crosses zero) are 40 ± 4.2, 43 ± 6.9, 22.6 ± 4.7, and 46.4 ± 8.3 for WT, ycgR, yhjH, and ycgR yhjH strains, respectively. The profiles are representative of 10 individual motors for each strain.