FIG 3.

Effect of c-di-GMP-induced EPS and curli fimbria production on motility of Salmonella and E. coli, as measured by migration in soft agar. (A) Wild-type Salmonella enterica, E. coli, and their indicated mutant derivatives were inoculated at the center of 0.3% LB swim agar plates and incubated at 30°C for 8 h. (B) Wild-type E. coli and the indicated six EPS/fimbria mutants engineered into the yhjH ycgR strain were assayed as in panel A. Δ6× denotes all six mutations combined into one strain. A paired-sample t test determined that there was no statistically significant difference between the ycgR yhjH mutant and any of the additionally mutated strains.