TABLE 2.
Intracellular c-di-GMP levels of pseudorevertant strains
| Strain | Intracellular c-di-GMP concn (nM)a |
|---|---|
| Wild type | 450 ± 30 |
| ycgR yhjH mutant | 850 ± 20 |
| JP1836b | 800 ± 60 |
| JP1837 | 920 ± 30 |
| JP1838 | 940 ± 15 |
| JP1839 | 930 ± 150 |
| JP1844 | 870 ± 0.02 |
| JP1852 | 910 ± 17 |
| ycgR yhjH rssAB mutant | 800 ± 10 |
a
c-di-GMP concentrations were quantified using liquid chromatography coupled with tandem mass spectrometry as described in Materials and Methods. Samples were prepared and assayed in triplicate; means and standard deviations of the means are shown.
b
JP strains signify pseudorevertant isolates from the ycgR yhjH double mutant; see Table S1 in the supplemental material. A paired-sample t test was used to calculate P values of <0.05 when wild-type values were compared with any of the other listed strains.