FIG 3.

Mutation frequency analyses. (A) Mutation frequency analysis of MG1655 ΔmutT::kan strain harboring a plasmid (pBAD or pBAD_Eco_mutT or pBAD_Eco_ndk [refer to Table S2]). Mutation frequencies (using 4 replicates for each strain) were determined by dividing the number of colonies that appeared on the LB plus Amp plate containing rifampin by the corresponding viable counts obtained on the LB plus Amp plate. (B) The expression of plasmid-borne EcoMutT or EcoNDK in the MG1655 ΔmutT::kan strain was verified under the experimental conditions.