Fig. 3. Identification of MEF2-C responsive genomic sequences in target genes.

(A) Read depth of MEF2C ChIP-Seq in GM12878 B-cells. Reads are aligned either to the forward (blue) or reverse (green) strands. Lines above the coverage plot indicate peaks identified using the optimal Irreproducible Discovery Rate (IDR).²³ The AGO3 gene contains two peaks, labeled 1 and 2. (B) Reporter constructs containing the putative MEF2C binding sites were transfected into WT and MEF2C KO cells and assayed for luciferase activity. Results are normalized to ß-gal activity. P-value above bars. NS=non-significant.