Table 1. Primers used to amplify four putative genes by real-time PCR.
Gene | Primer | Amplicon length |
---|---|---|
PqE |
F:5-GAACAGGCAGTTGGACAC R:5-GTCAGCACGGAGAACATC |
85 bp |
PqTS1 |
F:5-TTGGTGCTGTTGCTACTT R:5-AATCATCCTCCTCATTATCATACT |
81 bp |
PqTS2 |
F:5-GTCACAACATACAACAAGAA R:5-GCGAGATACACAACTAAGA |
107 bp |
PqTS3 |
F:5-CACAATGAACTGATACAAG R:5-TGAACTGATGAAGAGAAC |
100 bp |
Actin |
F:5-AACATCGTTCTCAGTGGTGG R:5-CCAGACACTGTACTTCCTTTCA |
101 bp |
Actin: Gene used for internal control.