FIGURE 1.

Cadherin-7 protein is observed in migratory neural crest cells contributing to the trigeminal ganglion. Representative transverse sections taken at the axial level of the forming ophthalmic lobe of the trigeminal ganglion over four chick embryo stages (HH13–16) followed by immunohistochemistry for Cadherin-7 (green), HNK-1 (red, labels neural crest cells), and Annexin A6 (purple, labels placodal neurons). (a–d) Lower magnification images show the entire transverse section and reveal Cadherin-7 immunoreactivity, with rostral and caudal orientation, the location of the forebrain (FB), hindbrain (HB), and ectoderm (e), and the ophthalmic lobe of the trigeminal (opV), indicated in (a) and applicable to (b–d). Asterisk (*) in (a–d) reveals Cadherin-7 immunoreactivity in the neural tube. Higher magnification images (a′–d′) of the boxed area in (a–d) show Cadherin-7-and HNK-1-double-positive neural crest cells at all stages (arrows), whereas Annexin A6-positive placodal neurons are devoid of Cadherin-7 (arrowheads). DAPI (blue) labels cell nuclei. Scale bar in (a) is 100 μm and applicable to (b–d), while scale bar in (a′) is 5 μm and applicable to (b′–d′)