Overexpression of Cadherin-7 effectively increases Cadherin-7 protein in neural crest cells contributing to the trigeminal ganglion. (a) Premigratory neural crest cells were electroporated at 2–3ss with either a Cadherin-7 expression construct (pCIG-Cad7) to allow for overexpression of Cadherin-7 protein in migratory neural crest cells, or the control vector (pCIG). Embryos were re-incubated HH15–17 after which time the trigeminal ganglion-forming region on the electroporated side of the embryo was dissected out of the embryo and pooled for lysate preparation. Immunoblotting for Cadherin-7 and β-actin (control) was performed as in (Shah et al.,2017), with a representative immunoblot shown. (b) Overexpression efficiency was assessed as previously described (Shah et al., 2017), with graph indicating results of immunoblot analysis as determined by normalizing Cadherin-7 to β-actin and calculating the increase in this normalized ratio from that obtained for the pCIG-treated lysate (arbitrarily set to 1, n = 2). The mean and standard error of the mean are shown. A 200% increase in Cadherin-7 protein levels is noted in the pCIG-Cad7-treated lysate compared to the control pCIG-treated lysate