FIG. 5.
Quantification of the exosomes isolated from the cell culture medium using the microfluidic device. (a) Detection of exosomes (undiluted, serially diluted to 1:2, 1:4, 1:8, 1:16) isolated from the cell culture medium using the microfluidic device. The initial concentration was defined as 16 AU/μl, and 2 μl of exosome solution was used for each detection (R2 = 0.99). Images of the reaction chamber are presented at the bottom for each standard concentration. Scale bar: 1 mm. (b) Detection of exosomes isolated from the cell culture medium using the exosome quantitative kit. The initial concentration was defined as 16 AU/μl, and 2 μL of exosome solution was used for each detection (R2 = 0.95). (c) Comparison of the concentration of initial and handling by the microfluidic device samples. The concentration of the exosomes was quantified using commercial exosome quantitative kits. A two-sided Student t-test was used to analyze the statistical difference between the two groups. *** indicates statistical significance (p < 0.001). Error bars indicate standard deviation, and every sample was measured three times individually.