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. 2019 Dec 4;11(23):11609–11623. doi: 10.18632/aging.102563

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Copyright © 2019 Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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The actions of the NR2F1-AS1 knockdown in OS cells are neutralized by recovery of FOXA1. (A) The efficiency of pc-FOXA1 transfection was assessed via western blotting. *P < 0.05 vs. group pcDNA3.1 (empty-vector control). (B–F) HOS and U2OS cells were cotransfected with si-NR2F1-AS1 and either pc-FOXA1 or the empty pcDNA3.1 vector. The CCK-8 assay, flow cytometry, and Transwell migration and invasion assays were conducted to quantitate cellular proliferation, apoptosis, cell cycle distribution, migration, and invasion. *P < 0.05 vs. group si-NC. ^#P < 0.05 vs. group si-NR2F1-AS1+pcDNA3.1.