Table 2. Primer sequences of genes selected for analysis pro-inflammatory cytokines.
| Target gene | Accession number | Primer | Primer sequence (5′-3′) | Product size | Tm (°C) |
| IL-1β | NM_008361 | Forward | AATGCCACCTTTTGACAGTGAT | 132bp | 60 |
| Reverse | TGCTGCGAGATTTGAAGCTG | ||||
| IL-6 | NM_001314054 | Forward | AGGATACCACTCCCAACAGACC | 140bp | 60 |
| Reverse | AAGTGCATCATCGTTGTTCATACA | ||||
| IL-8 | NM_ 011339 | Forward | TTTCCACCGGCAATGAAG | 118bp | 59 |
| Reverse | TAGAGGTCTCCCGAATTGGA | ||||
| IL-18 | NM_008360 | Forward | GGCTGCCATGTCAGAAGACT | 239bp | 60 |
| Reverse | GTCTGGTCTGGGGTTCACTG | ||||
| TNF-a | NM_013693 | Forward | CACGTCGTAGCAAACCACC | 88bp | 59 |
| Reverse | TGAGATCCATGCCGTTGGC | ||||
| INF-β | NM_010510 | Forward | GGCTTCCATCATGAACAACAGGT | 167bp | 61 |
| Reverse | AGGTGAGGTTGATCTTTCCATTCAG | ||||
| β-actin | NM_007393 | Forward | GCTGTGCTATGTTGCTCTAG | 117bp | 59 |
| Reverse | CGCTCGTTGCCAATAGTG |