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. 2019 Dec 20;10:2932. doi: 10.3389/fmicb.2019.02932

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Copyright © 2019 Brahim Belhaouari, Baudoin, Gnankou, Di Pinto, Colson, Aherfi and La Scola.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transmission electron microscopy of cellulase-treated P. massiliensis particles. (A1–A5) Control condition with untreated P. massiliensis particles. (B1–B5) P. massiliensis particles treated with cellulase solution diluted at 1:100, (B3–B5) particles showed a defect of the envelope. (C1–C5) P. massiliensis particles treated with cellulase solution diluted at 1:10, (C3–C5) particles exhibited detachments of the different layers of the envelope. (D1–D5) P. massiliensis particles treated with cellulase solution stock. (D3–D5) Particles imaged in different stages of digestion from least to the most digested. (D5) “Ghost-like” particles presenting totally empty internal spaces and only a thin surrounding tegument (black arrow).