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. 2019 Dec 20;10:2907. doi: 10.3389/fmicb.2019.02907

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Copyright © 2019 King, Siboni, Kahlke, Green, Labbate and Seymour.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of amplicon sequenced phylogenetic markers for the Vibrio mock communities. Mock communities 1, 2 and 3 are (A), (B) and (C) respectively. Each bar represents the average across a biological triplicate for each sequencing assay. Communities were characterized using 16S rRNA V3-V4 (Herlemann et al., 2011), Vibrio-specific 16S rRNA (Thompson et al., 2004; Yong et al., 2006), and Vibrio-centric hsp60 primer pairs. The true mock community composition is also shown. Displayed data are relative abundance summarized at the species level. For sequences assigned to the second chromosome (group 2), they were combined with their respective species. Sequences representing less than 1% of the relative abundance were removed.