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. 2019 Dec 20;7:334. doi: 10.3389/fcell.2019.00334

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Copyright © 2019 Rodriguez-Jimenez, Clemente, Moreno-Manzano and Erceg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD24 ependymal cell marker expression in GFAP-TK mouse spinal cord-derived neurospheres cultured in vitro and treated with 5-aza-dc or GCV. (A) GFAP-TK neurospheres were treated with vehicle [DMSO (V)], 5-aza-dc, or GCV. CD24 signal marks ependymal cells (red) while acetylated tubulin signal marks cilia (green). Pinwheel structures detected are marked with dashed lines indicating the periphery of ependymal cells and a solid white line indicating the core (schematic). Continue lines containing an asterisk mark the aberrant accumulation of acetylated tubulin. Scale bars, 20 μM. (B) Percentage of CD24 positive areas in 20 regions over multiple experiments (mean ± SD) were calculated for each condition using ImageJ software and compared to the total area. ^∗p < 0.05, ^∗∗p < 0.01 determined by Student’s t-test were considered statistically significant.