FIGURE 6.

GFAP astrocyte marker and CD24 ependymal cell expression in GFAP-TK mouse spinal cord-derived neurospheres cultured in vitro and treated with 5-aza-dc or GCV. (A) Cells treated with vehicle (DMSO [V]), 5-aza-dc, or GCV. Acetylated tubulin (green) antibody was used to mark cilia, a CD24 (red) antibody was used to mark ependymal cells, and a GFAP (blue) antibody was used to mark astrocytes, and all are shown in the merge. Central clusters are marked by a solid white line, and cores with aberrant accumulations of acetylated tubulin and GFAP detected after 5-aza-dc treatment are marked by asterisks and by a solid white line (schematic). CD24-positive ependymal cells and the pinwheel periphery are labeled by dashed lines (schematic). Astrocyte extensions that connect adjacent cores were labeled by white arrows (schematic). (B) Quantification of pinwheel structures in GFAP-TK mouse spinal cord-derived neurospheres cultured in vitro. The graphic displays the number of pinwheels counted per 100 total cells and from twenty pictures per condition after immunocytochemical evaluation. ^∗∗p < 0.01 determined by Student’s t-test was considered statistically significant.