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. 2019 Dec 20;7:431. doi: 10.3389/fbioe.2019.00431

Figure 8.

Figure 8

In vitro M1/M2 Macrophage differentiation assay. Monocytes were firstly isolated from peripheral blood cells by plastic adherence. The M1 differentiation was performed in the presence of 50 ng/ml of human Granulocyte-Macrophages Colony-Stimulating Factor (GM-CSF). The M2 differentiation was performed in the presence of 50 ng/ml of human Macrophages Colony-Stimulating Factor (GM-CSF). M1-differentiated Macrophages (MΦ-M1) and M2-differentiated Macrophages (MΦ-M2) were analyzed at day 6 of differentiation. In parallel, EV-endMSCs and IFNγ/EV-endMSCs were added to monocytes at day 0 and in vitro cultured for 6 days. Similarly, monocytes were differentiated toward M1 Macrophages (MΦ-M1) in the presence of EV-endMSCs and IFNγ/EV-endMSCs. The macrophages were then trypsinized and the surface expression of CD206 (M2 marker) was determined by flow cytometry in CD14+ cells. The lower boundary of the boxes indicates the 25th percentile and the upper boundary, the 75th percentile. Bars above and below the boxes indicate the 90th and 10th percentiles. The line within the boxes marks the median. No significant differences were observed between EV-endMSCs and IFNγ/EV-endMSCs. *p ≤ 0.05.