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. 2019 Dec 20;13:567. doi: 10.3389/fncel.2019.00567

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Copyright © 2019 Lin, Zhu, Ni, Rui, Sha, Yang, Li and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Double-immunofluorescence staining images of Nrf2 (red) with NeuN (green)-marked neurons (A) and Nrf2 (red) with GFAF (green)-marked astrocytes (C) to show the expression profiles in the sham group, vehicle group and 2-BFI group. Nuclei were counterstained with DAPI (blue) in the same view for each section. Immunofluorescence analysis was used to detect the IOD of Nrf2 signals in neurons (B) and astrocytes (D) at day 3 after the operation. Scale bars are 10 μm. Immunofluorescence intensities were determined using ImageJ software. n = 6 animals per group. Columns represent the mean ± SD. **P < 0.01 vs. the sham group; ^##P < 0.01 vs. the SCI+vehicle group.