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. 2019 Dec 20;7:345. doi: 10.3389/fcell.2019.00345

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Copyright © 2019 Akbari, Arslan, Senturk and Erdal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Key protocols for generating iPSC-derived 3D liver organoid cultures. FCS, Fetal Calf Serum; 3D, three-dimensional; FGF, Fibroblast Growth Factor; TGF, Transforming Growth Factor; R-SPO, R-Spondin; EGF, Epidermal Growth Factor; HGF, Hepatocyte Growth Factor; CM, Condition Medium; FACS, Fluorescence-Activated Cell Sorting; EpCAM, Epithelial Cell Adhesion Molecule; iPSC, Induced Pluripotent Stem Cell; BMP, Bone Morphogenetic Protein; ME, Mesoendoderm; HP, Hepatic Progenitor; KGF, Keratinocyte Growth Factor; HB, Hepatoblast; HCM, Hepatocyte Culture Medium; OSM, Oncostatin M; H/C, Hepatocyte/Cholangiocyte; Dex, Dexamethasone; ITS, Insulin-Transferrin-Selenium; DE, Definitive Endoderm; FG, Foregut; FP, Foregut Progenitor; CP, Cholangiocyte Progenitor.