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. 2019 Dec 20;12:316. doi: 10.3389/fnmol.2019.00316

FIGURE 7.

FIGURE 7

Validation of Esrrb regulon. (A) Gene set activity plot displays Esrrb regulon expression in scRNA-Seq dataset. The more red a dot is, the greater the regulon activity in that given cell. (B) Esrrb binding motifs for Esrrb downstream targets (Abcg1, Atp13a5, Heyl). (C) Feature plots demonstrating expression of Esrrb and Atp13a5 in the marginal cell cluster in the scRNA-Seq dataset. Maximum expression (normalized counts) is noted in the black outlined gray box for each gene in the lower left corner of each feature plot. Histogram is depicted from 0 to maximum (Max) in terms of normalized counts. Feature plots for Abcg1 and Heyl have been shown previously in Figure 3. (D) smFISH demonstrates co-expression of Abcg1, Atp13a5, and Heyl transcripts in red with Esrrb transcripts in green in marginal cells on representative cross-sections of the P30 mouse SV. Panels to left depict Esrrb transcripts (green) and downstream target transcripts (red) with DAPI. Panels to the right depict Esrrb transcripts (green) and downstream target transcripts (red) without DAPI. DAPI (4′,6-diamidino-2-phenylindole). All scale bars 20 μm.