KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat anti C-peptide | DSHB | Cat#GN-ID4; RRID: AB_2255626 |
| Rabbit anti Chaga | Abcam | Cat#Ab15160; RRID: AB_301704 |
| Chicken anti GFP | Aves Labs inc. | Cat#GFP-1020; RRID: AB_10000240 |
| Goat anti glp2 | Santa Cruz Biotechnology | Cat#sc-7781; RRID: AB_2107346 |
| Mouse anti Glucagon | Santa Cruz Biotechnology | Cat#sc-514592; RRID: AB_2629431 |
| Goat anti Neurod1 | R&D systems | Cat#AF2746; RRID: AB_2149217 |
| Sheep anti Neurogenin3 | R&D systems | Cat#AF3444; RRID: AB_2149527 |
| Mouse anti Nkx6.1 | DSHB | Cat#F55A12; RRID: AB_532379 |
| Rabbit anti Sox9 | Abcam | Cat#Ab-5535; RRID: AB_2239761 |
| Goat anti PDX1 | R&D systems | Cat#AF2419; RRID: AB_355257 |
| Mouse anti somatostatin | Santa Cruz Biotechnology | Cat#sc-55565; RRID: AB_831726 |
| Rabbit anti MCM3 | Cell Signaling Technology | Cat#4012s; RRID: AB_2235150 |
| Mouse anti Ctnnb1 | ThermoFisher Scientific | Cat#MA1–2001; RRID: AB_326078 |
| Goat anti Patched1 | Santa Cruz Biotechnology | Cat#sc-6147; RRID: AB_2253263 |
| Rat anti PTCH | R&D systems | Cat#MAB41051; RRID: AB_2174045 |
| Rabbit anti APC | Abcam | Cat#Ab-15270; RRID: AB_301806 |
| Mouse anti E-cadherin | BD Transduction Laboratories | Cat#610181; RRID: AB_397580 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| ActivinA | R&D Systems | Cat#338-AC |
| Rock Inhibitor Y-27632 | DNSK | Cat#DNSK-Kl15–02 |
| Chir99021 | Stemgent | Cat#04–0004-10 |
| KGF | Peprotech | Cat#100–19 |
| Sant1 | Sigma-Aldrich | Cat#S4572 |
| PDBu | EMD Millipore | Cat#524390 |
| XXI | EMD Millipore | Cat#565790 |
| Alk5i II | Axxora | Cat#ALX-270–445 |
| T3 | EMD Millipore | Cat#642511 |
| Betacellulin | ThermoFisher Scientific | |
| IWR1-endo | Sigma-Aldrich | Cat#I0161 |
| XAV939 | Santa Cruz Biotechnology | Cat#sc-296704A |
| Recombinant Human BMP2/BMP-7 Heterodimer | ThermoFisher Scientific | Cat#3229-BM |
| Critical Commercial Assays | ||
| ViewRNA ISH Tissue Assay Kit (2-plex) | ThermoFisher | Cat#QVT0012 |
| Anti human SMO ViewRNA probeset | ThermoFisher | Cat#VA1 −14719 |
| Anti human PTCH1 ViewRNA probeset | ThermoFisher | Cat#VA6–20367 |
| SMARTer® Ultra Low Kit for the Fluidigm® C1 System | Clontech | Cat#634833 |
| C1 Single-Cell Auto Prep Array for mRNA Seq (17–25 μm) | Fluidigm | Cat#100–5761 |
| Deposited Data | ||
| Raw and analyzed single cell sequencing data | This paper | SRA BioProject PRJNA532884 |
| Experimental Models: Cell Lines | ||
| HUES8hESCS | HSCI | hES Cell line: HUES-8 |
| hiPSC 1016 | HSCI | hiPSC 1016 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6j-ApcMin/J | Jackson Laboratories | RRID:IMSR_JAX:002020 |
| Mouse: B6.Cg-Apctm2Rak/Nci | NCI Mouse Repository | RRID:IMSR_NCIMR:01XAA |
| Mouse: Tg(Pdx1-cre)89.1Dam | In house | RRID:MGI:5902759 |
| Mouse: Tg(Neurog3-cre)C1Able/J | Jackson Laboratories | RRID:IMSR_JAX:005667 |
| Mouse: B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J | Jackson Laboratories | RRID:IMSR_JAX:006148 |
| Software and Algorithms | ||
| Topic modeling | This paper | http://groups.csail.mit.edu/cgs/geneprogram.html |
| Interactive database | This paper | https://ifx.rc.fas.harvard.edu/invitrobetacells/ |