Mean (±SEM) number of LH pulses (A), mean LH concentrations (B), LH pulse amplitude (C), blood glucose concentrations (D). A-C) Open bars and data points indicate pre-injection sampling period, filled bars and data points indicate post-injection period (n=6 or 7/group). D) Δ Fed + Saline, ○ Fasted + Saline, ● Fasted + Insulin. Unique letters signify significant differences between values (P < 0.05).