Fig. 6.

Noise exposure has no significant effect on cAMP levels in outer hair cells (OHCs), whereas treatment with forskolin increases cAMP expression in OHCs assessed 1 h after noise exposure. (A) Representative images of immunolabeling for cAMP (red) in OHCs of surface preparations of the upper basal turn. Inset enlarged images are for visualization of labeling. Green: phalloidin, scale bar = 10 μm. (B) Semi-quantification of immunolabeling for cAMP in grayscale in OHCs revealed an increase after treatment with forskolin but not after noise exposure. Data are presented as means +SD. The number of animals in each group is indicated in the bar graph; one cochlea was used per mouse, ns: non-significant, **p < 0.01, ***p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)