Table 1.
The table shows a list of primer indicating species, gene names, product size, Tm (temperature), and primer sequences (F: forward, R: reverse).
Species | Gene | Product size (bp) | Tm (℃) | Sequence (F) | Sequence (R) |
---|---|---|---|---|---|
Mouse | GAPDH | 113 | 60.11 | 5′-gagagtgtttcctcgtcccg-3′ | 5′-caatctccactttgccactgc-3′ |
CD4 | 105 | 59.89 | 5′-ccagacagtgttcctggctt-3′ | 5′- tgcctggcgctgttgg-3′ | |
TNF-α | 185 | 59.53 | 5′-ggcctccctctcatcagttc-3′ | 5′-cacttggtggtttgctacgac-3′ | |
IL-6 | 130 | 59 | 5′-agtccggagaggagacttca-3′ | 5′-gccattgcacaactcttttctca-3′ |