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. 2019 Sep 4;27(1):261–270. doi: 10.1016/j.sjbs.2019.09.004

Table 1.

The table shows a list of primer indicating species, gene names, product size, Tm (temperature), and primer sequences (F: forward, R: reverse).

Species Gene Product size (bp) Tm (℃) Sequence (F) Sequence (R)
Mouse GAPDH 113 60.11 5′-gagagtgtttcctcgtcccg-3′ 5′-caatctccactttgccactgc-3′
CD4 105 59.89 5′-ccagacagtgttcctggctt-3′ 5′- tgcctggcgctgttgg-3′
TNF-α 185 59.53 5′-ggcctccctctcatcagttc-3′ 5′-cacttggtggtttgctacgac-3′
IL-6 130 59 5′-agtccggagaggagacttca-3′ 5′-gccattgcacaactcttttctca-3′