TABLE 1.
Primer Pair | Sequence (5′ to 3′)b | Product size/bp |
Spa146f/Spa525r | AACAATGCGATYCCAGTATCRAG/CTACGACATTAAAGGTACCDCGG | 424 |
Spa93f/Spa525r | TCCGYCGTGGGATTGAGACC/CTACGACATTAAAGGTACCDCGG | 474 |
aThe two primer pairs consist of three primers, two forward primers Spa146f and Spa93f, and one reverse primer Spa525r. bIUPAC ambiguity codes: R (A or G), Y (C or T), D (G or A or T).