FIGURE 7.

SIV DNA positive cells detected by in situ hybridization in LTNP with undetectable viremia are shown in brown for mesenteric LN (BA11, A), spleen (CA66, B), inguinal LN (AL78, C), and colon (DT83, D). Similarly, RNA positive cells are shown in brown for colon (BA69, E,F) from a LTNP animal with undetectable viremia. All animals had undetectable plasma viral load (<125 copies/ml of plasma) at the time of euthanasia. Blue arrows in each figure indicate representative SIV-DNA/RNA positive cells. Panels (E,F) show RNA+ cells in a macaque (BA69), some of which are clearly in the telophase stage of cell division (red arrow). Note, that despite the animals had undetectable plasma viral load, several tissues had productively infected cells as well as DNA positive cells detected at the time of euthanasia.