Figure 3.

EET suppresses TGFβ1‐induced atrial fibroblasts activation. A, Representative images of atrial fibroblasts transdifferentiation with TGFβ1, EET, EEZE, GW9662 and SiRNA Smad‐7 (α‐SMA staining, scale bar: 250 μm). B, Statistical analysis of transdifferentiation ratio in atrial fibroblasts (10 visible areas/group; five independent experiments). C, Representative immunoblot of collagen‐I, collagen‐III, Smad‐2/3 phosphorylation and Smad‐7. D, Statistical analysis of relative expression of collagen‐I, collagen‐III and Smad‐2/3 phosphorylation, n = 3/group. E, Statistical analysis of relative expression of Smad‐7, n = 3/group. Three independent experiments for Western blot, *P < .05, compare with atrial fibroblasts without TGFβ1 stimulation; ^# P < .05, compare atrial fibroblasts with TGFβ1 stimulation; ^& P < .05, compare with atrial fibroblasts with TGFβ1 stimulation and EET treatment