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. 2019 Nov 20;24(1):862–874. doi: 10.1111/jcmm.14796

Figure 4.

Figure 4

CYP2J2/EET inhibits atrial fibrosis and atrial fibroblasts activation by targeting MiR‐21/Smad‐7. A, The effects of AAV9‐CYP2J2 on MiR‐21 expression in mice with or without AAC; *P < .05, compare with control group; # P < .05, compare with AAC group; & P < .05, compare with AAC + CYP2J2 group, n = 10/group. B, The effects of EET on MiR‐21 expression in atrial fibroblasts with or without TGFβ1 stimulation, n = 5/group. C, Representative images of atrial fibroblasts transdifferentiation with TGFβ1, EET, pre‐MiR‐21 (α‐SMA staining, scale bar: 250 μm). D, Statistical analysis of transdifferentiation ratio in atrial fibroblasts (10 visible areas/group; five independent experiments). E, Representative immunoblot of collagen‐I, collagen‐III, Smad‐2/3 phosphorylation and Smad‐7. F, Statistical analysis of relative expression of collagen‐I, collagen‐III and Smad‐2/3 phosphorylation. G, Statistical analysis of relative expression of Smad‐7. Three independent experiments for Western blot, *P < .05, compare with atrial fibroblasts without TGFβ1 stimulation; # P < .05, compare atrial fibroblasts with TGFβ1 stimulation; & P < .05, compare with atrial fibroblasts with TGFβ1 stimulation and EET treatment