Figure 4.

The effect of HOTAIR on the viability and apoptosis of ovarian granulosa cells in PCOS rat models. Note: A, the expression of HOTAIR in the ovarian granulosa cells detected by RT‐qPCR; B, viability of ovarian granulosa cells in response to oe‐HOTAIR and si‐HOTAIR detected by CCK‐8 assay; C, apoptosis of ovarian granulosa cells in response to oe‐HOTAIR and si‐HOTAIR detected by flow cytometry; D, quantitative analysis for the apoptosis rate of ovarian granulosa cells; **P < .01 compared with the blank group; *P < .05 compared with the blank group; PCOS, polycystic ovaries syndrome; RT‐qPCR, reverse transcription‐quantitative polymerase chain reaction; and CCK‐8, cell count kit‐8