Table 1.
Gene | Sequence |
---|---|
HOTAIR | F: 5’‐CATGGATCCACATTCTGCCCTGATTTCCGGAACC‐3’ |
R: 5’‐ACTCTCGAGCCACCACACACACACAACCTACAC‐3’ | |
miR‐130a | F: 5’‐GCTCTTTTCACATTGTGCTACT‐3’ |
R: 5’‐CAGTGCAATGTTAAAAGGGCATA‐3’ | |
IGF1 | F: 5’‐ATCTCTTCTACCTGGCACTCTG‐3’ |
R: 5’‐GAAGCAACACTCATCCACAAT‐3’ | |
GAPDH | F: 5’‐ACGGCAAGTTCAACGGCACAG‐3’ |
R: 5’‐GACGCCAGTAGACTCCACGACA‐3’ | |
U6 | F: 5’‐CTCGCTTCGGCAGCACA‐3’ |
R: 5’‐ACGCTTCACGAATTTGCGT‐3’ |
Abbreviations: F, forward; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; IGF1, insulin like growth factor 1; miR‐130a, microRNA‐130a; R, reverse; RT‐qPCR, reverse transcription‐quantitative polymerase chain reaction.