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. 2019 Nov 16;24(1):451–464. doi: 10.1111/jcmm.14753

Table 1.

Primer sequences for RT‐qPCR

Gene Sequence
HOTAIR F: 5’‐CATGGATCCACATTCTGCCCTGATTTCCGGAACC‐3’
R: 5’‐ACTCTCGAGCCACCACACACACACAACCTACAC‐3’
miR‐130a F: 5’‐GCTCTTTTCACATTGTGCTACT‐3’
R: 5’‐CAGTGCAATGTTAAAAGGGCATA‐3’
IGF1 F: 5’‐ATCTCTTCTACCTGGCACTCTG‐3’
R: 5’‐GAAGCAACACTCATCCACAAT‐3’
GAPDH F: 5’‐ACGGCAAGTTCAACGGCACAG‐3’
R: 5’‐GACGCCAGTAGACTCCACGACA‐3’
U6 F: 5’‐CTCGCTTCGGCAGCACA‐3’
R: 5’‐ACGCTTCACGAATTTGCGT‐3’

Abbreviations: F, forward; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; IGF1, insulin like growth factor 1; miR‐130a, microRNA‐130a; R, reverse; RT‐qPCR, reverse transcription‐quantitative polymerase chain reaction.