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. 2019 Oct 28;24(1):562–572. doi: 10.1111/jcmm.14764

Figure 3.

Figure 3

BAG3 reduction is a protective response of cells to glucose insufficiency. A, immunoblot analysis of ectopic BAG3 in HCT116 cells infected with lentivirus containing empty or BAG3 construct. B, viable cell count of HCT116 cells cultured under 1mM glucose. C, apoptotic cell analysis of HCT116 cells. D, immunoblot analyses of p53 and p21 protein in HCT116 cells. E, cell cycle analysis of HCT116 cells. F, immunoblot analysis of ectopic BAG3 in MCF7 and Bel‐7402 cells. (G‐H), viable cell count of MCF7 (G) and Bel‐7402 (H) cells cultured under 1mM glucose. (I‐J), cell cycle analysis of MCF7 (I) and Bel‐7402 (J) cells. K, immunoblot analysis of BAG3 expression in control or BAG3 knock in (KI) MEF cells. L, crystal violet assay for MEF cells. M, cell cycle analysis of MEF cells