Figure 5.

BAG3 promotes calpain‐dependent p53 degradation under metabolic stress. A, real‐time RT‐PCR analysis of p53 mRNA in HCT116, MCF7 and Bel‐7402 cells. B, immunoblot analysis of p53 in HCT116 and MEF cells cultured with 1 mmol/L glucose for the indicated time. C immunoblot analysis of p53 in the presence of protein degradation inhibitors (MG132, E64D and pepstatin A) or protein synthesis inhibitor cycloheximide (CHX) in the indicated cells. (D‐F), HCT116 (D), MEF (E), MCF7 and Bel‐7402 (F) cells were treated with 25 and 1 mmol/L glucose, immunoblot analyses were performed using the indicated antibodies. G, immunoblot analysis of p53 in HCT116 cells cultured under 1mM glucose in the presence of calpain inhibitor EDTA and Z‐LLY‐FMK