Figure 5.

Effect of JSH‐23 on pro‐inflammatory effect of NR4A3 overexpression in rat chondrocytes. NR4A3‐overexpressed or control chondrocytes were treated with or without NF‐κB inhibitor JSH‐23 (10 μmol/L) before IL‐1β stimulation (10 ng/mL). (A) mRNA level of NR4A3, INOS, COX2 and MMP9 was detected by real‐time PCR (similar results were obtained from five repeated experiments). (B) Protein level of NR4A3, INOS, COX2 and MMP9 was evaluated by Western blot analysis (similar results were obtained from 3 repeated experiments). *P < .05