Figure 6.

Effect of NR4A3 knockdown on IL‐1β‐induced NF‐κB and MAPK pathway activation in rat chondrocytes. (A) NR4A3‐knockdown or control chondrocytes were incubated with IL‐1β (10 ng/mL) for 10 min, and the NF‐κB pathway was evaluated by detecting the phosphorylation of its key effector p65 and degradation of IKB‐α, while activation of the MAPK pathway was investigated by detecting the phosphorylation of p38, Erk and JNK through Western blot analysis (similar results were obtained from 3 repeated experiments). (B) Nuclear translocation of p65 was visualized by immunofluorescence analysis (similar results were obtained from three repeated experiments); yellow arrows were used to illustrate inhibited nuclear translocation of p65. (C) Activation of NF‐κB was also detected by luciferase reporter gene analysis (similar results were obtained from five repeated experiments). *P < .05