Figure 1.

Artesunate inhibited glioma cell growth. A, The effects of artesunate on glioma cell growth were assessed by crystal violet staining. U87, U251, U138 and SK‐N‐SH cells were seeded in a 24‐well plate at a density of 1000 cells/well. Cells were treated with 20 µm or 100 µm artesunate for 14 d. Then, the cells were stained with a crystal violet solution. B, The effects of artesunate on glioma cell apoptosis were assessed by Annexin V staining, and the results were quantified. C, The effects of artesunate on glioma cell motility were assessed with a wound healing assay, and the results were quantified. D, The effects of artesunate on the anchorage‐independent growth of glioma cells were assessed with a soft agar assay. Scale bar, 100 µm. *P < .05; **P < .01