Figure 5.

LncRNA TNRC6C‐AS1 silencing promotes the apoptosis and autophagy of TC cells through STK4/Hippo. A, The mRNA expression of Beclin1 in transfected SW579 cells normalized to GAPDH was determined by RT‐qPCR. B, Western blot analysis was used to determine the protein levels of Beclin1, LC3‐II/I and Casepase‐3 in SW579 cells normalized to GAPDH after transfection. C, The proliferation ability of SW579 cells in each group was tested by EdU assay. D, TUNEL method was used to detect the apoptosis rate of SW579 cells. E, MDC method was used to detect the number of autophagic vesicles of SW579 cells in each group. *P < .05 vs the blank group, #P < .05 vs the shSTK4 group. The comparison among multiple groups was analysed by one‐way ANOVA (mean ± standard deviation). Values were obtained from 3 independent experiments in triplicate