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Primer sequences
| Gene | Primer sequence (5′‐3′) |
|---|---|
| STK4‐Methylation | F: GGATTTAGGATTTAGGTAGAGGTGC |
| R: CCTAATCACGTAATTCGAACGTA | |
| STK4‐Non‐Methylation | F: TTTAGGATTTAGGTAGAGGTGTGT |
| R: CCCTAATCACATAATTCAAACATA |
Abbreviations: F, forward primer; R, reverse primer; STK4, serine/threonine‐protein kinase4.
