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. 2019 Nov 13;24(1):441–450. doi: 10.1111/jcmm.14749

Figure 4.

Figure 4

LncRNA DLEU2 acted as a sponge of miR‐30a‐5p in NSCLC cells. A, Schematic representation of the binding sites between miR‐30a‐5p and WT or Mut lncRNA DLEU2. B, Luciferase activity of WT or Mut DLEU2 after the cotransfection with the miR‐30a‐5p mimic or inhibitor and WT or Mut DLEU2 vector in A549 and NCI‐H460 cells. C, qRT‐PCR analysis of the effects of lncRNA DLEU2 on the expression levels of miR‐30a‐5p in A549 and NCI‐H460 cells. D, RIP analysis of the amount of lncRNA DLEU2 and miR‐30a‐5p pulled down from the Ago2 protein in A549 and NCI‐H460 cells (E and F). MTT analysis of the proliferation viability after cotransfection with DLEU2 plasmid and miR‐30a‐5p mimic or sh‐DLEU2 and miR‐30a‐5p inhibitor in A549 and NCI‐H460 cells. Data shown are the mean ± SEM of three experiments. *P < .05